Identifi cation of ABO, RH and KEL blood group antigens with serology and genotyping methods

Introduction . Blood transfusion is a strong practice in traumatology, internal medicine, haematology, obstetrics and transplantation, which demands safety of haemotransfusion with estimating the red blood cell group antigens donor recipient blood. Routine immunotyping techniques usually provide for an antigen identification to weak subgroups, albeit certain inherent limitations serology tests that can be overcome genotyping approach. Aim — performance assessment methods ABO, RH KEL identification. Materials A total 55,489 1,898 patient samples have been analysed. Ambiguous cases chimerism, panagglutination inconsistent results were tackled genotyping. Serology performed gel cards. Whole DNA extraction was Qiagen chemistry. Allele-specific PCR used erythrocyte BAG Diagnostics commercial kits 2% agarose product detection. Sanger sequencing complement Results combined use allowed successful antigen-based Rh-status assignment 26 donors patients ambiguous typing. Conclusion Genotyping coupled serologic advised hampered